Lead in PDB 2g0a: X-Ray Structure of Mouse Pyrimidine 5'-Nucleotidase Type 1 with Lead(II) Bound in Active Site

All present enzymatic activity of X-Ray Structure of Mouse Pyrimidine 5'-Nucleotidase Type 1 with Lead(II) Bound in Active Site:
3.1.1.5;

The structure of X-Ray Structure of Mouse Pyrimidine 5'-Nucleotidase Type 1 with Lead(II) Bound in Active Site, PDB code: 2g0a was solved by E.Bitto, C.A.Bingman, G.E.Wesenberg, G.N.Phillips Jr., Center Foreukaryotic Structural Genomics (Cesg), with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	67.27 / 2.35
Space group	P 32
Cell size a, b, c (Å), α, β, γ (°)	134.549, 134.549, 39.226, 90.00, 90.00, 120.00
R / Rfree (%)	14.9 / 22.9

The binding sites of Lead atom in the X-Ray Structure of Mouse Pyrimidine 5'-Nucleotidase Type 1 with Lead(II) Bound in Active Site (pdb code 2g0a). This binding sites where shown within 5.0 Angstroms radius around Lead atom.
In total 2 binding sites of Lead where determined in the X-Ray Structure of Mouse Pyrimidine 5'-Nucleotidase Type 1 with Lead(II) Bound in Active Site, PDB code: 2g0a:
Jump to Lead binding site number: 1; 2;

Lead binding site 1 out of 2 in the X-Ray Structure of Mouse Pyrimidine 5'-Nucleotidase Type 1 with Lead(II) Bound in Active Site


Mono view


Stereo pair view

A full contact list of Lead with other atoms in the Pb binding site number 1 of X-Ray Structure of Mouse Pyrimidine 5'-Nucleotidase Type 1 with Lead(II) Bound in Active Site within 5.0Å range: probe atom residue distance (Å) B Occ A:Pb903 b:40.8 occ:0.25 OD2 A:ASP49 2.0 42.9 1.0 OD1 A:ASP238 2.1 42.8 1.0 O A:HOH905 2.3 19.0 1.0 OD2 A:ASP242 2.8 35.5 1.0 CG A:ASP49 3.2 38.5 1.0 O A:HOH1098 3.3 36.9 1.0 OD1 A:ASP242 3.3 30.0 1.0 CG A:ASP238 3.3 40.6 1.0 CG A:ASP242 3.4 32.1 1.0 O A:HOH914 3.5 25.4 1.0 OG A:SER239 3.6 38.7 1.0 O A:HOH970 3.7 36.6 1.0 OD2 A:ASP238 3.9 43.9 1.0 O A:HOH1256 4.0 48.9 1.0 OD1 A:ASP49 4.0 36.7 1.0 CB A:SER239 4.1 35.4 1.0 CB A:ASP49 4.2 35.7 1.0 N A:ASP238 4.3 36.1 1.0 N A:SER239 4.3 36.2 1.0 O A:HOH1283 4.3 60.6 1.0 NZ A:LYS213 4.5 36.5 1.0 CB A:ASP238 4.5 37.9 1.0 C A:ASP238 4.6 36.6 1.0 CA A:ASP238 4.6 37.2 1.0 O A:ASP51 4.7 38.7 1.0 CB A:ASP242 4.8 31.6 1.0 CA A:SER239 4.9 36.1 1.0 OG1 A:THR53 4.9 36.4 1.0 O A:HOH917 5.0 36.6 1.0

Lead binding site 2 out of 2 in the X-Ray Structure of Mouse Pyrimidine 5'-Nucleotidase Type 1 with Lead(II) Bound in Active Site


Mono view


Stereo pair view

A full contact list of Lead with other atoms in the Pb binding site number 2 of X-Ray Structure of Mouse Pyrimidine 5'-Nucleotidase Type 1 with Lead(II) Bound in Active Site within 5.0Å range: probe atom residue distance (Å) B Occ B:Pb904 b:39.4 occ:0.25 OD2 B:ASP49 1.9 41.8 1.0 OD1 B:ASP238 2.2 44.7 1.0 O B:HOH905 2.3 19.9 1.0 OD2 B:ASP242 2.7 32.5 1.0 O B:HOH1071 2.9 38.8 1.0 O B:HOH1013 3.1 34.3 1.0 CG B:ASP49 3.1 37.5 1.0 CG B:ASP238 3.3 40.7 1.0 OD1 B:ASP242 3.4 29.0 1.0 CG B:ASP242 3.4 31.3 1.0 O B:HOH920 3.5 22.3 1.0 OG B:SER239 3.7 39.0 1.0 O B:HOH1253 3.8 48.6 1.0 OD2 B:ASP238 3.8 43.6 1.0 OD1 B:ASP49 3.9 36.1 1.0 O B:HOH982 3.9 42.3 1.0 CB B:ASP49 4.2 35.7 1.0 CB B:SER239 4.2 36.0 1.0 N B:ASP238 4.3 36.4 1.0 N B:SER239 4.4 36.0 1.0 CB B:ASP238 4.5 38.2 1.0 O B:HOH1195 4.5 61.2 1.0 NZ B:LYS213 4.5 35.6 1.0 O B:ASP51 4.6 38.4 1.0 C B:ASP238 4.6 36.6 1.0 CA B:ASP238 4.7 37.1 1.0 CB B:ASP242 4.8 30.8 1.0 OG1 B:THR53 4.9 36.8 1.0 O B:HOH915 4.9 30.1 1.0 CA B:SER239 4.9 36.0 1.0

E.Bitto, C.A.Bingman, G.E.Wesenberg, J.G.Mccoy, G.N.Phillips. Structure of Pyrimidine 5'-Nucleotidase Type 1. Insight Into Mechanism of Action and Inhibition During Lead Poisoning. J.Biol.Chem. V. 281 20521 2006.
ISSN: ISSN 0021-9258
PubMed: 16672222
DOI: 10.1074/JBC.M602000200